Author/Authors :
Qujeq، Durdi نويسنده Department of Biochemistry & Biophysics, Babol University of Medical Sciences, Babol, Iran. , , Tatar ، Mohsen نويسنده Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran Tatar , Mohsen , feizi، Farideh نويسنده , , Parsian، Hadi نويسنده Department of Biochemistry & Biophysics, Babol University of Medical Sciences, Babol, Iran. , , Halalkhor ، Sohrab نويسنده Department of Biochemistry & Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran ,
Abstract :
Background and Aim: The purpose of this study is to evaluate effects of
Teucrium polium (TP) leaf extracts on adenosine monophosphate activated protein
kinase (AMPK) level in isolated pancreases.
Methods: In this experimental study, two groups of rats were used as a control
group, and diabetic group. In the end of experimental period the animals were
sacrificed then pancreas was removed, then isolated rat pancreas was cultured in
buffer with or without TP leaf extracts. Measuring the sample glucose level was
carried out by spectrophotometric method (Jenway, Model 6505, UK, Pars Azmmon
Co., Tehran, IRAN). Insulin level was assayed by an enzyme immunoassay
ELISA kit specific for rats made by Mercodia Rat insulin ELISA (10-1250-01,
Mercodia AB, Uppsala, Sweden). The adenosine monophosphate activated protein
kinase level was determined by ELISA assay method using a rat phosphorylated
AMPK ELISA kit (CSB-E11337r, Cusabio Biotech Co., LTD).
Results: The results indicate that addition of TP leaf extract significantly
increased AMPK level and insulin content compared to untreated group (p < 0.05).
Conclusion: These results support the potential of TP leaf extract as a therapeutic
modality for diabetes. However, further studies will be necessary to confirm this
potential
