Abstract :
A micro-sequential injection spectrophotometric procedure for DNA assay was developed based on the employment of a labon-valve (LOV) meso-fluidic analytical system. A small amount of crystal violet solution (10 (mu)l) was de-colored inside the flow cell of the LOV at the presence of 5 (mu)l (lambda)-DNA/HindIII within a certain pH range, and the absorbance decrease of crystal violet solution at 591 nm was measured via optical fibers and was employed as the basis of quantification. A uni-variant approach was adopted for the optimization of experimental parameters, including buffer pH, concentration and volume of crystal violet solution, reaction time and sample/reagent loading flow rates. A linear calibration graph was obtained within 0.2-6.0 (mu)g ml-1, along with a detection limit of 0.07 (mu)g ml-1. The procedure was applied for the determination of (lambda)-DNA/HindIII in synthetic samples in comparison with a documented procedure.
