Title of article
Studies of a viral suppressor of RNA silencing p19-CFP fusion protein: A FRET-based probe for sensing double-stranded fluorophore tagged small RNAs
Author/Authors
Roger Koukiekolo، نويسنده , , Zygmunt J. Jakubek، نويسنده , , Jenny Cheng، نويسنده , , Selena M. Sagan، نويسنده , , John Paul Pezacki، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2009
Pages
4
From page
166
To page
169
Abstract
Eukaryotes have evolved complex cellular responses to double-stranded RNA. One response that is highly conserved across many species is the RNA silencing pathway. Tombusviruses have evolved a mechanism to evade the RNA silencing pathway that involves a small protein, p19, that acts as a suppressor of RNA silencing. This protein binds specifically to small-interfering RNAs (siRNAs) with nanomolar affinity in a sequence-independent manner and with size selectivity.
Keywords
RNA silencing , Carnation Italian ringspot virus , Chemical probes , RNA–protein interactions , FRET
Journal title
Biophysical Chemistry
Serial Year
2009
Journal title
Biophysical Chemistry
Record number
1120222
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