Title of article :
Stabilizing effect of polyols is sensitive to inherent stability of protein Original Research Article
Author/Authors :
Md. Zahid Kamal، نويسنده , , Shoeb Ahmad، نويسنده , , Nalam Madhusudhana Rao، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Abstract :
In studies on polyol-mediated protein stabilization, the polyols are the preferred variable and less importance is given to the intrinsic properties of the protein used. We investigated the stabilizing effects of glycerol on three in vitro evolved lipase mutants with varying stabilities and also in a broad pH range of 3.3–12.1. Significant linear negative correlation between increment in stability due to glycerol and prior stability suggests that stabilizing effects of glycerol depend on the prior stability of the protein. Polar/nonpolar surface area and charge do not have a bearing on the stabilizing effects of glycerol.
Keywords :
Lipase , protein stability , Polyol , Glycerol , in vitro evolution
Journal title :
Biophysical Chemistry
Journal title :
Biophysical Chemistry