Title of article
Biosynthesis of the Angiogenesis Inhibitor Borrelidin by Streptomyces parvulus Tü4055: Cluster Analysis and Assignment of Functions Original Research Article
Author/Authors
Carlos Olano، نويسنده , , Barrie Wilkinson، نويسنده , , César S?nchez، نويسنده , , Steven J Moss، نويسنده , , Rose Sheridan، نويسنده , , Vidya Math، نويسنده , , Alison J Weston، نويسنده , , Alfredo F Bra?a، نويسنده , , Christine Saint-Martin، نويسنده , , Markiyan Oliynyk، نويسنده , , Carmen Méndez، نويسنده , , Peter F Leadlay، نويسنده , , José A Salas، نويسنده ,
Issue Information
ماهنامه با شماره پیاپی سال 2004
Pages
11
From page
87
To page
97
Abstract
The biosynthetic gene cluster for the angiogenesis inhibitor borrelidin has been cloned from Streptomyces parvulus Tü4055. Sequence analysis indicates that the macrolide ring of borrelidin is formed by a modular polyketide synthase (PKS) (borA1-A6), a result that was confirmed by disruption of borA3. The borrelidin PKS is striking because only seven rather than the nine modules expected for a nonaketide product are encoded by borA1-A6. The starter unit of the PKS has been verified as trans-cyclopentane-1,2-dicarboxylic acid (trans-1,2-CPDA), and the genes involved in its biosynthesis identified. Other genes responsible for biosynthesis of the nitrile moiety, regulation, and self-resistance were also identified.
Journal title
Chemistry and Biology
Serial Year
2004
Journal title
Chemistry and Biology
Record number
1158763
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