Title of article :
RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function Original Research Article
Author/Authors :
Qian Li، نويسنده , , Yunkyung Kim، نويسنده , , Joshua Namm، نويسنده , , Amita Kulkarni، نويسنده , , Gus R. Rosania، نويسنده , , Young-Hoon Ahn، نويسنده , , Young Tae Chang، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2006
Pages :
9
From page :
615
To page :
623
Abstract :
The higher-order structural organization of the cell nucleus reflects the underlying genome-wide transcriptional activity and macromolecular transport processes. To study the microscopic organization of RNA distribution within the nucleus, a combinatorial library of fluorescent styryl molecules was synthesized and screened for an in vitro RNA response and live cell nuclear imaging. Four different cell lines (HeLa, A549, 3T3, and 3T3-L1) were analyzed in terms of higher-order nuclear organization. We identified RNA-selective dyes with better imaging properties relative to commercially available SYTORNASelect dye; the selected dyes were also cell permeant, photostable, and well tolerated by the cells. Our dyes also had very good counterstain compatibility with Hoechst and DAPI, which could help to image the DNA distribution in relation to RNA distribution in live cells and therefore reveal different patterns of RNA-DNA colocalization.
Journal title :
Chemistry and Biology
Serial Year :
2006
Journal title :
Chemistry and Biology
Record number :
1159215
Link To Document :
بازگشت