Inactivation of lipase by carbon dioxide under atmospheric pressure Original Research Article

Lipase exposed to CO2 (under atmospheric pressure) treatment at different pH and temperature conditions showed a decrease in enzyme activity. Inactivation was found to be highest as the temperature increased from 20 to 50 °C at initial pH 7.15. About 84% of the initial activity of the enzyme is lost at initial pH 7.15 and 50 °C. The faster drop in pH and enzyme activity occurred within 5 min. Without CO2, no significant change (P>0.05) was observed in activity at the highest temperature (50 °C). The pH drop and loss in enzyme activity were higher at initial pH 8.20. No changes in enzyme activity and pH were observed when nitrogen gas was applied instead of CO2. Studies using gel electrophoresis showed no differences in protein patterns between CO2-treated and non-treated control. After 6 weeks of frozen storage, no changes in pH and activity were observed for CO2-treated lipase solution.