Performance of immobilized Pectinex Ultra SP-L on magnetic duolite-polystyrene composite particles Part I: a batch reactor study Original Research Article

Pectinex Ultra SP-L was immobilized on magnetic duolite-polystyrene particles. Immobilization and reaction conditions were determined by a viscometric method in the batch system reactor. Maximum free and immobilized activities of the enzyme were both obtained at 65 °C and pH 6. Activation energies for free and immobilized enzymes were calculated as 4.39 and 6.83 kcal mole−1 respectively. The kinetics of free and Duolite A568-immobilized pectinase was investigated. Michaelis–Menten constants and maximal reaction rates were found as Km=0.515 g ml−1 and Vmax=1.047×10−3 g μl−1 s−1 for free enzyme and Km=1.877 g ml−1 and Vmax=5.569×10−3 g s−1 g particles−1 for immobilized pectinase at 20 °C.