High-Producers of Polygalacturonase Selected From Mutants Resistant to Rifampin in Alkalophilic Bacillus sp. NTT33

Synthesis of polygalacturonases in alkalophilic Bacillus sp. which is sensitive to rifampin, was clearly repressed by glucose. Mutants resistant to glucose catabolic repression were selected. From spontaneous mutants, NTT33-cs52 and NTT33-cs301 produced polygalacturonase activities 57.2 to 82.4% higher than the wild type.