Title of article :
Immobilization of cycloisomaltooligosaccharide glucanotransferase for the production of cycloisomaltooligosaccharides from dextran
Author/Authors :
Hiroshi Kawamoto، نويسنده , , Tetsuya Oguma، نويسنده , , Hiroshi Sekine، نويسنده , , Mikihiko Kobayashi ، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Pages :
7
From page :
515
To page :
521
Abstract :
Immobilization of cycloisomaltooligosaccharide glucanotransferase (CITase) and its application in the production of cycloisomaltooligosaccharides (CIs) from dextran were studied. Among various carrier materials examined, the enzyme adsorbed physically on Chitopearl BCW-3505 showed the highest activity (1.75 U/ml carrier). The activity remaining was 35%. The maximum CI yield in batch reactions at 0.2, 2 and 10% dextran was 28, 24 and 12%, respectively. The maximum CI yield at 2% dextran (24%) was slightly less than that with the free enzyme under the same conditions (26%). The concentration of linear oligosaccharides, the byproducts in the reaction mixture, was greater with the immobilized CITase than the free enzyme. The immobilized CITase was less thermostable than the free enzyme by about 10°C. The pattern of influence of Ca2+ concentration on the thermostability differed between the free and immobilized CITase. A Ca2+ concentration of 50–100 mM was optimum for the thermostability of the immobilized CITase, 10–50 mM for the free enzyme. CIs were produced continuously by a column system packed with the immobilized enzyme at 40°C with a space velocity (SV) of 6 h−1. The three quarters life time was 4 weeks. We think that relatively long life time at fast SV was accomplished and CI production cost by this method should be lower than the batch reaction. This is the first report on immobilization of CITase.
Keywords :
Immobilization , CI , CITase , Cycloisomaltooligosaccharide glucanotransferase , Cycloisomaltooligosaccharide
Journal title :
Enzyme and Microbial Technology
Serial Year :
2001
Journal title :
Enzyme and Microbial Technology
Record number :
1173408
Link To Document :
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