Title of article :
An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate
Author/Authors :
Sunita Teotia، نويسنده , , S.K. Khare، نويسنده , , M.N. Gupta، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Abstract :
β-amylases are used in production of maltose syrup. It is shown that sweet potato β-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean and barley enzymes indicate that this may be a general method for purification of β-amylases.
Keywords :
Alginate , Enzyme purification , ?-amylase , Affinity precipitation , Sweet potato
Journal title :
Enzyme and Microbial Technology
Journal title :
Enzyme and Microbial Technology