Production of glycerol by fermentation using osmophilic yeast Candida krusei with different starchy substrates

An osmophilic yeast, Candida Krusei, was used for glycerol production with various starchy substrates. The results showed that cassava starch and corn starch were easily liquefied and saccharified compared with corn meal and sweet potato meal. The optimal concentrations of corn steep liquor (CSL) added to the liquefied and saccharified media from corn meal, sweet potato meal, cassava starch, and corn starch were 0, 0, 0.15%, and 0.1%, respectively. Correspondingly, the maximal glycerol productions were 3.8%, 3.5%, 5.5%, and 7.1%, respectively. Furthermore, it was found that glycerol produced was utilized as the second carbon source at 24 h, 24 h, 72 h, and 84 h, respectively. in addition, accompanying the lower rate of glycerol production (RGP), the maximal glycerol production (GP) and glycerol yield (GY) were reached when corn starch was selected as starchy substrate. in the case of corn meal and sweet potato used as starchy substrates, biomass increased rapidly and residual glucose decreased quickly, compared with corn starch and cassava starch.