Biocatalytic resolution of dl-pantolactone by glutaraldehyde cross-linked cells of Fusarium moniliforme CGMCC 0536

Fusarium moniliforme CGMCC 0536 showing excellent stereoselective d-pantonohydrolase activity was immobilized by cross-linking with glutaraldehyde. Various characteristics of immobilized cells were assessed. Optimum conditions for enzyme activity were not affected after cross-linking, and the pH and temperature for both free and cross-linked cells were found to be pH 7.5 and 55 °C, respectively. The Km value of cross-linked cells (118 mM) was slightly higher than that of free cells (96 mM), while the Vmax value decreased from 4.18 to 3.87 mM min−1 g−1 wet cells after cross-linking. Compared with the free cells, the half-life of cross-linked cells was noticeably increased, and the operational stability of the biocatalyst for recycling in batch processes was markedly improved (no obvious decrease after 110 times).