Membrane assisted coupled enzyme system for phospholipid modification

Phospholipase D obtained from a culture of Streptomyces sp. strain PMF, was immobilised in a hollow fiber membrane by ultrafiltration. The reactor was used to contact an organic solution containing phosphatidylcholine (PC) and a water phase; the rate of formation of the hydrolysis product (phosphatidic acid, PA) depending on different parameters (pH, temperature, PC concentration and enzyme units) was measured. Subsequently, the water phase was replaced with a 2–3 M solution of different alcohols (l-serine, glycerol and ethanolamine) and the rate of transphosphatidylation was studied and finally optimized using glycerol as model alcohol.