Title of article
Hydroxylation of androstenedione by resting Rhodococcus sp. cells in organic media
Author/Authors
B. Angelova، نويسنده , , P. Fernandes، نويسنده , , A. Cruz، نويسنده , , H.M. Pinheiro، نويسنده , , S. Mutafov، نويسنده , , J.M.S. Cabral، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
5
From page
718
To page
722
Abstract
The use of organic solvent-based media for microbial 9α-hydroxylation of androstenedione (AD) by resting Rhodococcus sp. cells was evaluated. Product yields higher than 90% were observed in a two-phase phthalate-buffer transformation medium (3:1, v/v) containing 1.0 g L−1 AD. The results suggest that in these conditions the reaction of Δ1-dehydrogenation is considerably inhibited. The successful hydroxylation reaction with a product yield exceeding 60% was found to take place also in a phthalate medium under simultaneous forced aeration and maintenance of a minimum water content of about 3% (v/v).
Keywords
Bis-(2-ethylhexyl) phthalate , Microbial transformation , Rhodococcus , Steroids
Journal title
Enzyme and Microbial Technology
Serial Year
2005
Journal title
Enzyme and Microbial Technology
Record number
1174421
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