Induction of secondary metabolite production in transformed callus of Ammi majus L. grown after electromagnetic treatment of the culture medium

High-performance liquid chromatography analysis of crude chloroform and methanol extracts as well as methanol preparations subjected to enzymatic hydrolysis indicated two-fold higher accumulation of umbelliferone in transformed Ammi majus L. callus grown on medium exposed to ADR-4® (energy stimulator plate) in comparison to the control cultures. Elicitation with ADR-4® induced also two times higher accumulation of bergapten. Three different techniques: Soxhlet extraction, ultrasonication and accelerated solvent extraction (ASE) applied for the extraction of coumarins, furanocoumarins and corresponding glycosides from transformed callus of A. majus elicited by ADR-4® plates were compared. ASE is shown to be the most efficient extraction method.