Title of article :
Enzymatic hydrolysis and fermentation of palm kernel press cake for production of bioethanol
Author/Authors :
José Mar?a Cerver?، نويسنده , , Pernille Anastasia Skovgaard، نويسنده , , Claus Felby، نويسنده , , Hanne Risbjerg S?rensen، نويسنده , , Henning J?rgensen، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
8
From page :
177
To page :
184
Abstract :
Palm kernel press cake (PKC) is a residue of palm oil extraction, which was found to contain 48.5% of total carbohydrates of which 35.2% was mannan. The present study examines enzymatic hydrolysis of polysaccharides from the cell-wall material present in PKC to obtain monosaccharides that can be substrate in various fermentation processes such as ethanol production. The requirements for pretreatment were investigated and it was found that mannan in PKC was readily hydrolysed without any pretreatment. Several enzyme preparations were tested and Mannaway 25L was found as the best for releasing mannose, and Gammanase 1.0L worked well in degrading cellulose and mannose. Binary mixtures of enzymes were tested to increase the conversion, and 1:1 mixture of Mannaway 25L and Gammanase 1.0L showed good synergistic effect releasing 30% more mannose than the sum obtained using these enzymes individually. Using an enzyme loading of 2.3 mg protein/g PKC resulted in 63% of mannan in PKC being hydrolysed to mannose in 24 h, and in 96 h a total of 365 g mannose and glucose could be produced per kg PKC. Finally, PKC was hydrolysed and fermented using Saccharomyces cerevisiae with an ethanol yield of 125 g/kg PKC.
Keywords :
Saccharomyces cerevisiae , Bioethanol , mannan , Palm kernel press cake
Journal title :
Enzyme and Microbial Technology
Serial Year :
2010
Journal title :
Enzyme and Microbial Technology
Record number :
1185526
Link To Document :
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