Title of article :
Development of biosensor for phenolic compounds containing PPO in β-cyclodextrin modified support and iridium nanoparticles
Author/Authors :
Cristiano P. da Silva، نويسنده , , Ana C. Franzoi، نويسنده , , Suellen C. Fernandes، نويسنده , , Jairton Dupont، نويسنده , , Iolanda C. Vieira، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Pages :
6
From page :
296
To page :
301
Abstract :
A biosensor based on the iridium nanoparticles dispersed in ionic liquid (IL) 1-butyl-3-methylimidazolium hexafluorophosphate (Ir-BMI·PF6) and a celery (Apium graveolens) extract as a source of polyphenol oxidase (PPO) was constructed. A modified support based on β-cyclodextrin (β-CDEP) was used for enzyme immobilization. The behavior of phenolic compounds was investigated by square-wave voltammetry and rutin was selected by presenting the greatest signal. The best performance was obtained with a composition of 70:10:10:10% (w/w/w/w) of the graphite powder:β-CDEP:Nujol:Ir-BMI·PF6 composition, a PPO concentration of 500 units mL−1, in 0.1 M phosphate buffer solution (pH 6.0) with frequency, pulse amplitude and scan increment at 100 Hz, 60 mV, and 3.0 mV, respectively. Under optimized conditions, the cathodic currents increased linearly for the rutin concentration range of 1.3 × 10−7–2.0 × 10−6 M with a detection limit of 7.9 × 10−8 M. This sensor demonstrated acceptable repeatability and reproducibility and the results for the rutin recovery ranged from 92.8 to 103.4%. A relative error of 0.7% was obtained in the rutin determination in simulated samples.
Keywords :
Biosensor , Rutin , Ionic liquid , Nanoparticles , Polyphenol oxidase
Journal title :
Enzyme and Microbial Technology
Serial Year :
2013
Journal title :
Enzyme and Microbial Technology
Record number :
1186007
Link To Document :
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