Abstract :
The effect of 15,30 and 60 atm CO2pressure at 25,35 and 45°C on Listeria monocytogenes was studied. A biphasic curve was observed in the CO2treatments. A primary curve was characterized with very little killing and a secondary curve was showed straight-line inactivation kinetics. An increase of pressure and/or temperature enhanced the antimicrobial effects of CO2. Listeria monocytogenes suspended in physiological saline containing 1% brain–heart infusion broth was completely inactivated under 60 atm CO2treatment in 115,75 and 60 min at 25,35 and 45°C, respectively. A minimum D -value was obtained under 60 atm CO2pressure at 45°C. High pressure CO2treatment technique can possibly be applied to reduce microbiol load in whole and skim milk; and carrot, orange, peach, and apple juices.
