Rapid DNA preparation from milk and dairy process samples for the detection of bacteria by PCR

In this communication, a rapid, easy pretreatment of milk and dairy process samples is presented in order to detect the microbes present in the samples by the polymerase chain reaction (PCR). It includes the use of commercial membrane cards for the disruption of cells and the storage of DNA, and the inclusion of a card punch in a PCR as the template source. Successful detection of starter, probiotic and pathogen strains in dairy food samples was obtained in PCR by using the membrane-based method for DNA preparation.