Title of article
A Simplified Procedure of Enzyme Extraction for the Assay of Polygalacturonase and Cellulase in Some Fruits
Author/Authors
SAIPRASAD، G. V. S. نويسنده , , MYIHILI، J. B. نويسنده , , NAGESH، M. نويسنده , , JOSI، R. نويسنده , , RAVISHANKAR، K. V. نويسنده , , ANAND، LALITHA نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
-296
From page
297
To page
0
Abstract
A simplified procedure involving extraction of homogenised tissue in 1.0 M sodium acetate buffer followed by 48 h dialysis against distilled water has been standardized for the assay of polygalacturonase and cellulase activities of mango, tomato and papaya. The results indicated that satisfactory enzyme activities could be obtained by using a one step extraction procedure without adjusting pH and overnight stirring. This procedure could, therefore, be used effectively to process large number of samples.
Keywords
mango , tomato , ripening , cellulase , Polygalacturonase , Enzyme extraction , Enzyme assay , Papaya
Journal title
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
Serial Year
2004
Journal title
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
Record number
119627
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