A Simplified Procedure of Enzyme Extraction for the Assay of Polygalacturonase and Cellulase in Some Fruits

A simplified procedure involving extraction of homogenised tissue in 1.0 M sodium acetate buffer followed by 48 h dialysis against distilled water has been standardized for the assay of polygalacturonase and cellulase activities of mango, tomato and papaya. The results indicated that satisfactory enzyme activities could be obtained by using a one step extraction procedure without adjusting pH and overnight stirring. This procedure could, therefore, be used effectively to process large number of samples.