Author/Authors :
Mihaljevic، I نويسنده Agricultural Institute Osijek, Ju?no predgra?e 17, HR-31000 Osijek, Croatia , , Dugalic، Krunoslav نويسنده Agricultural Institute Osijek, Ju?no predgra?e 17, HR-31000 Osijek, Croatia , , STEHLIK-TOMAS، VESNA نويسنده , , Viljevac، Marija نويسنده Agricultural Institute Osijek, Ju?no predgra?e 17, HR-31000 Osijek, Croatia , , Pranjic، Ankica نويسنده Agricultural Institute Osijek, Ju?no predgra?e 17, HR-31000 Osijek, Croatia , , CMELIK ، Zlatko نويسنده , , Puskar، Boris نويسنده Agricultural Institute Osijek, Ju?no predgra?e 17, HR-31000 Osijek, Croatia , , Jurkovic، Zorica نويسنده Agricultural Institute Osijek, Ju?no predgra?e 17, HR-31000 Osijek, Croatia ,
Abstract :
Surface sterilization is the most important step in preparation of
explants for micropropagation, because controlling fungal and bacterial
contamination of woody plant from field sources is very difficult. Six sterilizing
agents: sodium hypochlorite (NaOCl), calcium hypochlorite [Ca(ClO)2], sodium
dichloroisocyanurate (DICA), mercuric (II) chloride (HgCl2), silver nitrate
(AgNO3) and hydrogen peroxide (H2O2) were tested for sterilization of
‘Obla?inska’ sour cherry buds, by varying their concentration and time of
exposure. The aim of this study was to establish best surface sterilization for in
vitro propagation of ‘Obla?inska’ sour cherry. Aseptic cultures of ‘Obla?inska’
sour cherry were established from axillary buds which were placed in nutrient
medium, supplemented with plants hormones 6-benzylaminopurine (BA), 1-
naphthaleneacetic acid (NAA) and gibberellic acid (GA3). The results indicated
that among these sterilizing agents silver nitrate (AgNO3) at concentration of 1%
for 20 minutes was the best for controlling the infection, whereas sterilization with
sodium dichloroisocyanurate (DICA) at concentration of 1% for 10 minutes was
not satisfactory.
