Title of article :
The influence of molecular mass of sulfated propylene glycol ester of low-molecular-weight alginate on anticoagulant activities
Author/Authors :
Chang-Zheng Lin، نويسنده , , Huashi Guan، نويسنده , , Hai-Hua Li، نويسنده , , Guangli Yu، نويسنده , , Cai-Xia Gu، نويسنده , , Guoqiang Li، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Pages :
7
From page :
3009
To page :
3015
Abstract :
Sulfated propylene glycol ester of low-molecular-weight alginate (PSS) was fractionated by low-pressure gel permeation chromatography. Four fractions (PSS1–4) with different weight-averaged molecular mass (51.95, 25.62, 11.76 and 5.41 kD, respectively) were obtained and their structural characteristics were determined and compared by Fourier transform infrared and nuclear magnetic resonance spectroscopes. Their anticoagulant activities were also studied by evaluating their influence on the activated partial thromboplastin time, thrombin time, anti-thrombin and anti-FXa factor activities mediated by anti-thrombin III and heparin cofactor II. The results demonstrated that PSS1–4 had similar structural parameters, such as the degree and pattern of sulfation and backbone, but their molecular masses were different. The bioassay results suggested that the anticoagulation was molecular mass dependent. PSS1 and PSS2 strongly inhibited the activity of thrombin mediated by heparin cofactor II and anti-thrombin III, whereas PSS4 only showed a very weak anti-thrombin activity. PSS1–3 weakly inhibited the activity of coagulation factor Xa mediated only by anti-thrombin III while PSS4 lacked the anti-Xa activity.
Keywords :
Alginate , Sulfated propylene glycol alginate , anticoagulant , Coagulation factor
Journal title :
European Polymer Journal(EPJ)
Serial Year :
2007
Journal title :
European Polymer Journal(EPJ)
Record number :
1227268
Link To Document :
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