Title of article :
Bistable Secondary Structures of Small RNAs and Their Structural Probing by Comparative Imino Proton NMR Spectroscopy
Author/Authors :
Claudia Hobartner، نويسنده , , Ronald Micura، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
Abstract :
We investigate 25–34 nucleotide RNA sequences, that have been rationally designed to adopt two different secondary structures that are in thermodynamic equilibrium. Experimental evidence for the co-existence of the two conformers results from the NH⋯N 1H NMR spectra. When compared to the NH⋯N 1H NMR spectra of appropriate reference sequences the equilibrium position is easily quantifiable even without the assignment of the individual NH resonances. The reference sequences represent several Watson–Crick base-paired double helical segments, each encountered in either of the two conformers of the bistable target sequence. In addition, we rationalize the influence of nucleotide mutations on the equilibrium position of one of the bistable RNA sequences. The approach further allows a detailed thermodynamic analysis and the evaluation of secondary structure predictions for multistable RNAs obtained by computational methods.
Keywords :
imino protons , RNA folding , secondary structure , multistable RNAs , conformational equilibria
Journal title :
Journal of Molecular Biology
Journal title :
Journal of Molecular Biology