Determination of Ciprofloxacin by Flow Injection Analysis Based on Chemiluminescence System

A novel flow injection chemiluminescence (CL) method for the determination of ciprofloxacin (CPLX) was reported. The proposed method is based on luminescence produced by KMnO4-Na2S2O4-Tb3+-CPLX chemiluminescence (CL) system. The effects of some critical experimental conditions were discussed and the optimization of working conditions was investigated. The linear dynamic range is 3.00×10−8∼9.00×10−7 mol·L−1 and the detection limit for CPLX is 4.40 × 10−9 mol·L−1. This method was applied to the determination of CPLX in tablet, urine, and serum samples and the recoveries of real sample analyses were in the range from 110±4 to 104±4.