Author/Authors :
M. Salomo، نويسنده , , K. Kroy، نويسنده , , K. Kegler، نويسنده , , C. Gutsche، نويسنده , , M. Struhalla، نويسنده , , J. Reinmuth، نويسنده , , W. Skokov، نويسنده , , C. Immisch، نويسنده , , U. Hahn، نويسنده , , R. R. Netz and F. Kremer ، نويسنده ,
Abstract :
Optical tweezers are employed to study the action of the histone-like protein from Thermotoga maritima (TmHU) on DNA at a single molecule level. Binding and disruption of TmHU to and from DNA are found to take place in discrete steps of 4–5 nm length and a net binding enthalpy of about 16kBT. This is in reasonable agreement with a microscopic model that estimates the extension of the binding sites of the protein and evaluates the energetics mainly for bending of the DNA in the course of interaction.
Keywords :
protein–DNA interaction , optical tweezers , histone-like protein , Thermotoga maritima , single molecule study
