Title of article
Mammalian Mitochondrial DNA Replication Intermediates Are Essentially Duplex but Contain Extensive Tracts of RNA/DNA Hybrid
Author/Authors
Jaakko L.O. Pohjoism?ki، نويسنده , , J. Bradley Holmes، نويسنده , , Stuart R. Wood، نويسنده , , Ming Yao Yang، نويسنده , , Takehiro Yasukawa، نويسنده , , Aurelio Reyes ، نويسنده , , Laura J. Bailey، نويسنده , , Tricia J. Cluett، نويسنده , , Steffi Goffart، نويسنده , , Smaranda Willcox، نويسنده , , Rachel E. Rigby، نويسنده , , Andrew P. Jackson، نويسنده , , Johannes N. Spelbrink، نويسنده , , Jack D. Griffith، نويسنده , , Robert J. Crouch، نويسنده , , Howard T. Jacobs، نويسنده , , Ian J. Holt، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2010
Pages
12
From page
1144
To page
1155
Abstract
We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.
Keywords
DNA Replication , RNA/DNA hybrid , mitochondrial DNA , electrophoresis , Electron microscopy
Journal title
Journal of Molecular Biology
Serial Year
2010
Journal title
Journal of Molecular Biology
Record number
1251533
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