Fluorescence quenching of 3,7-diamino-2,8-dimethyl-5-phenyl phenazinium chloride by AgCl and Ag nanoparticles

Quenching of fluorescence of the dye 3,7-diamino-2,8-dimethyl-5-phenyl Phenazinium Chloride (Safranine T) has been investigated by AgCl nanoparticles in the W/O microemulsion medium at different [H2O]/[AOT] ratios (ω) and with Ag nanoparticles and Ag+ in aqueous medium. A simple straightforward method has been introduced to prepare AgCl nanoparticles in well-characterized, monodispersed biomimicking nanocavities formed by sodium bis(2-ethylhexyl)sulfosuccinate (AOT) in heptane. Experimental results reveal that the size of the AgCl nanoparticles increases with increase in hydration. The results of the quenching experiment were analysed in the light of Stern Volmer equation. Quenching of fluorescence of the dye has been found to decrease with decrease in the size of the nanoparticles of AgCl and the variation of Stern Volmer quenching constants (KSV) with particle size is different for two different size regimes.