Media Composition and Anther Plating for Production of Androgenetic Green Plants from Cultivated Rye (Secale cereale L.)

Media composition and anther plating environment were studied in rye anther culture. In the studies on media the effects of basal media composition, plant growth regulators, glutamine, sucrose and maltose, and solidification of media were investigated. Increase in the size of the induction dish improved regeneration of green plants. The basal media W14 and 190-2 were superior in promoting callus induction and green plant regeneration. Genotype by medium interactions for induction were not recorded. Dicamba auxin in the induction medium seemed beneficial for the green to albino plant ratio. Glutamine improved induction generally and green plant regeneration with a responsive genotype. Adding 10% ficoll type 400 was favourable for induction with one rye line, but this effect was not confirmed in subsequent testing with two different ryes. Solid medium promoted the highest production of green plants. Maltose in induction medium was superior to glucose and the maltose concentration of 6% seemed optimal for induction in comparison with higher concentrations and most cost-effective for production of green plants.