Cryopreservation of Chrysanthemum cinerariaefolium Shoot Tips

A simple method for efficient cryopreservation of Chrysanthemum cinerariaefolium shoot tips is described. It comprises the following steps: treatment of the explanted shoot tips with 0.55 mol/L sucrose plus 4 µmol/L ABA for 3 days, direct immersion in liquid nitrogen, rapid rewarming at 40 °C and recovery of the shoot tips on solid nutrient medium containing 11 µmol/L ANA and 4.5 µmol/L BAP About 75 % of the shoot tips withstood cryopreservation using this procedure. The morphogenic potential of frozen shoot tips, and total chlorophyll and pyrethrin contents of regenerated plants were preserved after the different treatments.