Title of article :
Mechanism of biochemical action of substituted 4-methylbenzopyran-2-ones. Part II: Mechanism-based inhibition of rat liver microsome-mediated aflatoxin B1–DNA binding by the candidate antimutagen 7,8-diacetoxy-4-methylcoumarin Original Research Article
Author/Authors :
Hanumantharao G. Raj، نويسنده , , Virinder S. Parmar، نويسنده , , Subhash C. Jain، نويسنده , , Sanjay Goel، نويسنده , , Amarjit Singh، نويسنده , , Kshitij Gupta، نويسنده , , Vishwajeet Rohil، نويسنده , , Yogesh K Tyagi، نويسنده , , Hirday N. Jha، نويسنده , , Carl E Olsen، نويسنده , , Jesper Wengel، نويسنده ,
Abstract :
7,8-Diacetoxy-4-methylcoumarin (DAMC), with no prerequisite for oxidative biotransformation has been reported to produce suicide inactivation of microsomal cytochrome P-450-catalysed formation of aflatoxin B1-8,9-oxide that binds to DNA. Parenteral administration of DAMC to rats caused significant inhibition of AFB1 binding to hepatic DNA in vivo as well as AFB1-induced micronuclei formation in bone marrow cells. These results highlight the antimutagenic potential of DAMC.
