Synthesis of an artificial hole-transporting nucleoside triphosphate, dMDATP, and its enzymatic incorporation into DNA Original Research Article

We report on the enzymatic synthesis of DNA wires by successive incorporation of artificial nucleotides. We synthesized an efficient hole-transporting nucleoside triphosphate, dMDATP, and have examined the screening of polymerases and reverse transcriptases for enzymatic incorporation of dMDATP into DNA. We found that KOD Dash is an effective enzyme for the enzymatic synthesis of MDA-containing DNA. Furthermore, the efficiency of enzymatic incorporation was remarkably improved by the addition of manganese chloride. By applying the PCR method to the synthesis of DNA containing a run of MDA, the MDA run was amplified up to 6 × 106 times. MDA runs prepared by the enzymatic method exhibited a high hole-transporting ability.