Title of article
A colorimetric assay for screening transketolase activity Original Research Article
Author/Authors
Mark E.B Smith، نويسنده , , Ursula Kaulmann، نويسنده , , John M. Ward، نويسنده , , Helen C. Hailes، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
4
From page
7062
To page
7065
Abstract
A tetrazolium red-based colorimetric assay has been devised to screen for transketolase activity with a range of aldehyde acceptors. The colorimetric TK assay is able to detect >8% bioconversion using non-α-hydroxylated aldehydes as acceptor substrates and is significantly faster and more convenient to use than chromatographic procedures.
Keywords
Colorimetric assay , Transketolase , directed evolution , Tetrazolium red
Journal title
Bioorganic and Medicinal Chemistry
Serial Year
2006
Journal title
Bioorganic and Medicinal Chemistry
Record number
1305197
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