HSP90-like artificial chaperone activity based on indole β-cyclodextrin Original Research Article

Indole β-cyclodextrin (β-1) was found to be able to prevent aggregation of citrate synthase (CS) on heating condition. As a result, β-1 showed anti-CS aggregation in this system by regulating in early stage. The depression mechanism of β-1 for aggregation of CS is as follows: the β-1 formed a complex with hydrophobic parts of the β-sheet structure of CS. From CD spectra, CS was changed own conformation was changed by β-1 addition. So, it was concluded that β-1 works as β-sheet inducer in thermal condition. On the other hand, native β-cyclodextrin (β-CyD) shows small suppression capability for CS aggregation.