Novel mannosyl derivatives of peptidoglycan monomer: Synthesis and biological evaluation of immunomodulatory properties Original Research Article

The aim of this work was to prepare mannosyl derivatives of peptidoglycan monomer (PGM, β-d-GlcNAc-(1→4)-d-MurNAc-l-Ala-d-isoGln-mesoDAP(εNH2)-d-Ala-d-Ala) in order to study the effects of mannosylation on adjuvant (immunostimulating) activity. Novel Man-OCH2CH(CH3)CO–PGM isomers were substrates for N-acetylmuramyl-l-alanine amidase, like the parent PGM molecule. Adjuvant activity of Man-OCH2CH(CH3)CO–PGM was tested in the mouse model using ovalbumin as an antigen.