Title of article
Site-specific labeling of ‘second generation’ annexin V with 99mTc(CO)3 for improved imaging of apoptosis in vivo Original Research Article
Author/Authors
Marijke de Saint Hubert، نويسنده , , Felix M. Mottaghy، نويسنده , , Kathleen Vunckx، نويسنده , , Johan Nuyts، نويسنده , , Humphrey Fonge، نويسنده , , Kristof Prinsen، نويسنده , , Sigrid Stroobants، نويسنده , , Luc Mortelmans، نويسنده , , Niko Deckers، نويسنده , , Leo Hofstra، نويسنده , , Chris PM Reutelingsperger، نويسنده , , Alfons Verbruggen، نويسنده , , Dirk Rattat، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2010
Pages
8
From page
1356
To page
1363
Abstract
In this study ‘second generation’ AnxV was specifically labeled with 99mTc in three different ways outside the binding region of the protein to obtain an improved target-to-background activity ratio. The compounds were tested in vitro and in vivo in normal mice and in a model of hepatic apoptosis (anti-Fas mAb). The apoptosis binding was most prominent for the HIS-tagged ‘second generation’ AnxV labeled with 99mTc(CO)3 in comparison to 99mTc-HYNIC-cys-AnxV and 99mTc(CO)3-DTPA-cys-AnxV.
Keywords
Annexin V , Technetium-99m , Imaging , Tricarbonyl labeling , apoptosis
Journal title
Bioorganic and Medicinal Chemistry
Serial Year
2010
Journal title
Bioorganic and Medicinal Chemistry
Record number
1307136
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