Luminescent nanocrystal metal organic framework based biosensor for molecular recognition

A nanocrystal metal organic framework (NMOF1) has been investigated for molecular recognition. NMOF1 {[Cd(atc)(H2O)2]n} has been synthesized by the reaction of Cd(II) ions with the sodium salt of ‘atc’ (‘atc’ = 2-aminoterephthalic acid) in aqueous medium at room temperature. Pendent single bondCOOH group of the organic linker was activated using EDC [(1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide] intermediated organic reaction, followed by the bioconjugation of NMOF1 with anti-BSA (anti-bovine serum albumin). NMOF1/anti-BSA complex has been used for the sensing of BSA (bovine serum albumin) based on the fluorescence quenching phenomena. Estimated limit of detection of the proposed molecular probe is 7 mg/mL.