Author/Authors :
Saha، s نويسنده Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh , , Khandoker، M.A.M.Y نويسنده , , Asad، L.Y نويسنده Asad, L.Y , Reza، A.M.M.T. نويسنده Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh , , Hoque، A. نويسنده Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh ,
Abstract :
This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II stage was 61.41 ± 1.97%. The matured COCs were fertilized for 5 hours in Brackett and Oliphant media using fresh and fro-zen thawed semen separately. After fertilization the oocytes were cultured in TCM-199 for 48 hours to ob-serve the cleavage rate. The maturation, fertilization and culture were performed in an incubator at 38.5 ?C with 5% CO2 in humidified air. After fertilization cleavage rates were observed to check the fitness of zy-gotes to be morula and blastocyst. It was observed that the rates of normal fertilization (2 PN formations) for fresh and frozen semen were 36.02 ± 2.79 and 34.73 ± 2.58%, respectively and the cleavage rates were 25.19 ± 2.5 and 21.01 ± 2.8%, respectively. No significant differences (P > 0.05) was observed between fresh and frozen semen in the efficiency of in vitro fertilization and subsequent development of goat em-bryos. It can be concluded that, both fresh and frozen semen can be used for IVF and subsequent develop-ment of goat embryos.
