Author/Authors :
Mu، نويسنده , , David and Chen، نويسنده , , Liyun and Zhang، نويسنده , , Xiping and See، نويسنده , , Lei-Hoon and Koch، نويسنده , , Christina M and Yen، نويسنده , , Clifford and Tong، نويسنده , , James Jiayuan and Spiegel، نويسنده , , Lori and Nguyen، نويسنده , , Ken C.Q and Servoss، نويسنده , , Allyson and Peng، نويسنده , , Yue and Pei، نويسنده , , Lin and Marks، نويسنده , , Jeffrey R and Lowe، نويسنده , , Scott and Hoey، نويسنده , , Timothy and Jan، نويسنده , , Lily Yeh and McCombie، نويسنده , , W.Richard and Wigler، نويسنده , , Michael H and Powers، نويسنده , , Scott، نويسنده ,
Abstract :
Representational difference analysis (RDA) of human breast cancer was used to discover a novel amplicon located at chromosomal region 8q24.3. We examined a series of breast cancer samples harboring amplification of this region and determined that KCNK9 is the sole overexpressed gene within the amplification epicenter. KCNK9 encodes a potassium channel that is amplified from 3-fold to 10-fold in 10% of breast tumors and overexpressed from 5-fold to over 100-fold in 44% of breast tumors. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation, suggesting that its amplification and overexpression plays a physiologically important role in human breast cancer.
