Author/Authors :
Szakلcs، نويسنده , , Gergely and Annereau، نويسنده , , Jean-Philippe and Lababidi، نويسنده , , Samir and Shankavaram، نويسنده , , Uma and Arciello، نويسنده , , Angela and Bussey، نويسنده , , Kimberly J. and Reinhold، نويسنده , , William and Guo، نويسنده , , Yanping and Kruh، نويسنده , , Gary D. and Reimers، نويسنده , , Mark and Weinstein، نويسنده , , John N. and Gottesman، نويسنده , , Michael M.، نويسنده ,
Abstract :
For analysis of multidrug resistance, a major barrier to effective cancer chemotherapy, we profiled mRNA expression of the 48 known human ABC transporters in 60 diverse cancer cell lines (the NCI-60) used by the National Cancer Institute to screen for anticancer activity. The use of real-time RT-PCR avoided artifacts commonly encountered with microarray technologies. By correlating the results with the growth inhibitory profiles of 1,429 candidate anticancer drugs tested against the cells, we identified which transporters are more likely than others to confer resistance to which agents. Unexpectedly, we also found and validated compounds whose activity is potentiated, rather than antagonized, by the MDR1 multidrug transporter. Such compounds may serve as leads for development.
