Title of article :
MOZ-TIF2, but not BCR-ABL, confers properties of leukemic stem cells to committed murine hematopoietic progenitors
Author/Authors :
Huntly، نويسنده , , Brian J.P. and Shigematsu، نويسنده , , Hirokazu and Deguchi، نويسنده , , Kenji and Lee، نويسنده , , Benjamin H. and Mizuno، نويسنده , , Shinichi and Duclos، نويسنده , , Nicky and Rowan، نويسنده , , Rebecca and Amaral، نويسنده , , Sonia and Curley، نويسنده , , David and Williams، نويسنده , , Ifor R. and Akashi، نويسنده , , Koichi and Gilliland، نويسنده , , D.Gary، نويسنده ,
Abstract :
To better understand the origin of leukemic stem cells, we tested the hypothesis that all leukemia oncogenes could transform committed myeloid progenitor cells lacking the capacity for self-renewal, as has recently been reported for MLL-ENL. Flow-sorted populations of common myeloid progenitors and granulocyte-monocyte progenitors were transduced with the oncogenes MOZ-TIF2 and BCR-ABL, respectively. MOZ-TIF2-transduced progenitors could be serially replated in methylcellulose cultures and continuously propagated in liquid culture, and resulted in an acute myeloid leukemia in vivo that could be serially transplanted. In contrast, BCR-ABL transduction conferred none of these properties to hematopoietic progenitors. These data demonstrate that some, but not all, leukemia oncogenes can confer properties of leukemic stem cells to hematopoietic progenitors destined to undergo apoptotic cell death.
