Author/Authors :
Shipitsin، نويسنده , , Michail and Campbell، نويسنده , , Lauren L. and Argani، نويسنده , , Pedram and Weremowicz، نويسنده , , Stanislawa and Bloushtain-Qimron، نويسنده , , Noga and Yao، نويسنده , , Jun and Nikolskaya، نويسنده , , Tatiana and Serebryiskaya، نويسنده , , Tatiana and Beroukhim، نويسنده , , Rameen and Hu، نويسنده , , Min and Halushka، نويسنده , , Marc K. and Sukumar، نويسنده , , Saraswati and Parker، نويسنده , , Leroy M. and Anderson، نويسنده , , Karen S. and Harris، نويسنده , , Lyndsay N. and Garber، نويسنده , , Judy E. and Richardson، نويسنده , , Andrea L. and Schnitt، نويسنده , , Stuart J. and Nikolsky، نويسنده , , Yuri and Gelman، نويسنده , , Rebecca S. and Polyak، نويسنده , , Kornelia، نويسنده ,
Abstract :
Summary
with distinct phenotypes including stem-cell-like properties have been proposed to exist in normal human mammary epithelium and breast carcinomas, but their detailed molecular characteristics and clinical significance are unclear. We determined gene expression and genetic profiles of cells purified from cancerous and normal breast tissue using markers previously associated with stem-cell-like properties. CD24+ and CD44+ cells from individual tumors were clonally related but not always identical. CD44+ cell-specific genes included many known stem-cell markers and correlated with decreased patient survival. The TGF-β pathway was specifically active in CD44+ cancer cells, where its inhibition induced a more epithelial phenotype. Our data suggest prognostic relevance of CD44+ cells and therapeutic targeting of distinct tumor cell populations.
