Author/Authors :
Pollard، نويسنده , , Patrick J. and Spencer-Dene، نويسنده , , Bradley and Shukla، نويسنده , , Deepa and Howarth، نويسنده , , Kimberley and Nye، نويسنده , , Emma and El-Bahrawy، نويسنده , , Mona and Deheragoda، نويسنده , , Maesha and Joannou، نويسنده , , Maria and McDonald، نويسنده , , Stuart and Martin، نويسنده , , Alison and Igarashi، نويسنده , , Peter and Varsani-Brown، نويسنده , , Sunita and Rosewell، نويسنده , , Ian and Poulsom، نويسنده , , Richard and Maxwell، نويسنده , , Patrick and Stamp، نويسنده , , Gordon W. and Tomlinson، نويسنده , , Ian P.M.، نويسنده ,
Abstract :
Summary
ne mutations in the fumarate hydratase (FH) tumor suppressor gene predispose to leiomyomatosis, renal cysts, and renal cell cancer (HLRCC). HLRCC tumors overexpress HIF1α and hypoxia pathway genes. We conditionally inactivated mouse Fh1 in the kidney. Fh1 mutants developed multiple clonal renal cysts that overexpressed Hif1α and Hif2α. Hif targets, such as Glut1 and Vegf, were upregulated. We found that Fh1-deficient murine embryonic stem cells and renal carcinomas from HLRCC showed similar overexpression of HIF and hypoxia pathway components to the mouse cysts. Our data have shown in vivo that pseudohypoxic drive, resulting from HIF1α (and HIF2α) overexpression, is a direct consequence of Fh1 inactivation. Our mouse may be useful for testing therapeutic interventions that target angiogenesis and HIF-prolyl hydroxylation.
