Author/Authors :
Muntean، نويسنده , , Andrew G. and Tan، نويسنده , , Jiaying and Sitwala، نويسنده , , Kajal and Huang، نويسنده , , Yongsheng and Bronstein، نويسنده , , Joel and Connelly، نويسنده , , James A. and Basrur، نويسنده , , Venkatesha and Elenitoba-Johnson، نويسنده , , Kojo S.J. and Hess، نويسنده , , Jay L.، نويسنده ,
Abstract :
Summary
involved in chromosomal rearrangements that generate fusion proteins with deregulated transcriptional activity. The mechanisms of MLL fusion protein-mediated transcriptional activation are poorly understood. Here we show MLL interacts directly with the polymerase associated factor complex (PAFc) through sequences flanking the CxxC domain. PAFc interacts with RNA polymerase II and stimulates posttranslational histone modifications. PAFc augments MLL and MLL-AF9 mediated transcriptional activation of Hoxa9. Conversely, knockdown of PAFc disrupts MLL fusion protein-mediated transcriptional activation and MLL recruitment to target loci. PAFc gene expression is downregulated during hematopoiesis and likely serves to regulate MLL function. Deletions of MLL that abolish interactions with PAFc also eliminate MLL-AF9 mediated immortalization indicating an essential function for this interaction in leukemogenesis.
