Title of article :
In Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal Rhabdomyosarcoma
Author/Authors :
Ignatius، نويسنده , , Myron S. and Chen، نويسنده , , Eleanor and Elpek، نويسنده , , Natalie M. and Fuller، نويسنده , , Adam Z. and Tenente، نويسنده , , Inês M. and Clagg، نويسنده , , Ryan and Liu، نويسنده , , Sali and Blackburn، نويسنده , , Jessica S. and Linardic، نويسنده , , Corinne M. and Rosenberg، نويسنده , , Andrew E. and Nielsen، نويسنده , , Petur G. and Mempel، نويسنده , , Thorsten R. and Langenau، نويسنده , , David M.، نويسنده ,
Abstract :
Summary
nal rhabdomyosarcoma (ERMS) is an aggressive pediatric sarcoma of muscle. Here, we show that ERMS-propagating potential is confined to myf5+ cells and can be visualized in live, fluorescent transgenic zebrafish. During early tumor growth, myf5+ ERMS cells reside adjacent normal muscle fibers. By late-stage ERMS, myf5+ cells are reorganized into distinct regions separated from differentiated tumor cells. Time-lapse imaging of late-stage ERMS revealed that myf5+ cells populate newly formed tumor only after seeding by highly migratory myogenin+ ERMS cells. Moreover, myogenin+ ERMS cells can enter the vasculature, whereas myf5+ ERMS-propagating cells do not. Our data suggest that non-tumor-propagating cells likely have important supportive roles in cancer progression and facilitate metastasis.
