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In molecular biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (qPCR) is a laboratory technique based on the PCR, which is used to amplify and quantify a targeted DNA molecule at the same time. For one or more specific sequences in a DNA sample, real-time PCR enables both detection and quantification. The quantity can be either an absolute number of copies or a relative amount when normalized to additional normalizing endogenous controls. Also, real-time PCR is combined with reverse transcription to quantify mRNA and non-coding RNA in cells or tissues.