Author/Authors :
KURD، NEMATULLAH نويسنده Department of Occupational Health Engineering, Hamedan University of Medical Sciences, Hamadan, Iran , , Bahrami ، Abdulrahman نويسنده Department of Occupational Health, School of Public Health, Hamadan University of Medical Science, Hamadan, Iran , , Ghorbani Shahna ، Farshid نويسنده Department of Occupational Health, School of Health, Hamedan University of Medical Sciences, Hamedan, Iran , , Heidari، Mahmoud نويسنده Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran ,
Abstract :
Acetonitrile used as a solvent in manufactures and affects to central nervous system from inhalation exposure. The aim of this study was to develop a micro-solid phase extraction method for the determination of acetonitrile in the air matrix. The sampling was performed with a small diameter fused silica fiber coated with a thin film of stationary phase and was subsequently desorbed and analyzed by gas chromatograph equipped with a flame ionization detector (GC/FID). The effects of laboratory and sampling parameters were investigated and applied to the determination of acetonitrile in air matrix. The Carboxen/PDMS as the coating fiber showed better analytical performances compared to the PDMS fiber. Analysis of the data by ANOVA test at a 0.05 level of accuracy showed that the peak area of the sampler was significantly affected by temperature and humidity so that the optimum temperature was 20°C and the optimum humidity was 35%. Besides, the limit of detection (LOD) and limit of quantification (LOQ) for acetonitrile in the GC system were 0.05 and 0.15 ?g/ml, respectively. The solid phase microextraction (SPME) has been shown a suitable technique for sampling and analysis of acetonitrile in air. There was a good correlation between the SPME and national institute occupational safety and health (NIOSH) 1010 method under the optimum conditions.
