A new matrix for membrane affinity chromatography and its application to the purification of concanavalin

Macroporous cellulose membranes with large pore sizes (1–2 μm) and high porosity (about 60%) were prepared from filter paper by chemical crosslinking. These membranes were activated by introducing epoxy groups with various spacer lengths, after which maltose was immobilized as an affinity ligand. Packed into a cartridge, the prepared membranes were used for the affinity purification of concanavalin A.