Author/Authors :
Nabavi، Narges نويسنده Department of Animal Science, Islamic Azad University, Damghan Branch, Damghan, Iran. , , Todehdehghan، Fatemeh نويسنده Department of Venomous Animals, Razi Vaccine and serum Research Institute, Karaj, Iran , , Shiravi، Abdollhossein نويسنده Department of Animal Science, Islamic Azad University, Damghan Branch, Damghan, Iran. ,
Abstract :
Background: Caffeine increases the CAMP production that stimulates spermatozoa movement. Caffeine is also used for induction of in vitro acrosome reaction in mammalian spermatozoa, an important step in achieving fertilization. Objective: The aim of this study was to assess the effect of caffeine on spermʹs motility, vitality and laboratory fertilization rates in mouse in two T6 and M16 media. Materials and Methods: Epididymal mouse sperms were collected and treated by caffine in T6 and M16 media and their motility and vitality rates were evaluated. The pretreated sperms were added to oocytes in T6 and M16 media with and without caffeine and fertilization rates were recorded after 24 hours incubation. Results: Spermʹs motility (81.7±1.67%) and vitality (88.7±1.33%) rates and percentage of fertilized oocytes (67.52±8.16%) in T6 medium plus caffeine compare to control group have increased and shown significant differences at p?0.01. While the percentages of these parameters in M16 medium supplemented with caffeine were 68.3±6.01%, 78±6.11%, and 42.6±12.96 respectively and in comparison to control group (M16 without caffeine) have not shown significant differences. Conclusion: Addition of caffeine to T6 medium promotes the spermʹs motility and vitality and enhances fertilization and early in vitro development of mouse embryos.
