Author/Authors :
Forootanfar، Hamid نويسنده 1Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran , Iran,Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, , , Moezzi، Atefeh نويسنده Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 14174, Iran , , Aghaie-Khozani، Marzieh نويسنده 1Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical SciencesTehranIran , , Mahmoudjanlou، Yasaman نويسنده 1Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical SciencesTehranIran , , Ameri، Alieh نويسنده , , Niknejad، Farhad نويسنده Faculty of Paramedicine and Health, Golestan University of Medical Sciences, Gorgan, IR Iran , , Faramarzi، Mohammad Ali نويسنده ,
Abstract :
Decolorization of six synthetic dyes using three sources of fungal laccase with the origin of Aspergillus oryzae,
Trametes versicolor, and Paraconiothyrium variabile was investigated. Among them, the enzyme from P. variabile was
the most efficient which decolorized bromophenol blue (100%), commassie brilliant blue (91%), panseu-S (56%),
Rimazol brilliant blue R (RBBR; 47%), Congo red (18.5%), and methylene blue (21.3%) after 3 h incubation in
presence of hydroxybenzotriazole (HBT; 5 mM) as the laccase mediator. It was also observed that decolorization
efficiency of all dyes was enhanced by increasing of HBT concentration from 0.1 mM to 5 mM. Laccase from A.
oryzae was able to remove 53% of methylene blue and 26% of RBBR after 30 min incubation in absence of HBT, but
the enzyme could not efficiently decolorize other dyes even in presence of 5 mM of HBT. In the case of laccase
from T. versicolor, only RBBR was decolorized (93%) in absence of HBT after 3 h incubation.
